ECOL3453 - Crop Ecology
Molecular Biology

In addition to genetic engineering, other molecular biology techniques are useful to plant breeders.

RFLPs

• Restriction Fragment Length Polymorphisms (RFLPs)
• RFLPs and corn Improvement
• Mapping the restorer gene Rf3 with RFLPs
• Assignment of RFLP linkage groups to rice chromosomes
• Uses of DNA technology in seed research

PCR

• Polymerase Chain Reaction - Xeroxing DNA
• What The Heck is PCR?
• The Polymerase Chain Reaction (PCR) Jump Station "The ultimate Web page for information and links on all aspects of the Polymerase Chain Reaction".
  

RAPDs

• Genetic similarity of new world and old world Saccharum assessed by RAPD analysis
• Development of Randomly Amplified Polymorphic DNA Markers Characteristic of Hibiscus rosa-sinensis and H. syriacus
• Identification of a RAPD-marker linked to the BaMMV/BaYMV resistance gene ym4
• Diffusion routes of buckwheat cultivation in Asia revealed by RAPD markers
• Genetics of growth rate and flowering time in papaya (Carica papaya L.)

AFLPs

• Vos et al., 1995
• An image explaining the AFLP procedure
• A catalogue on chromosome specific AFLP markers for alignment of potato maps
• RAPD and AFLP tagging of two genes .... which influence B-carotene accumulation in fruit of tomato.

Genome Mapping

• Agricultural Genome Information System (AGIS)
• The National Corn Genome Initiative
• The building blocks of grass
• Saccharum spontaneum genetic linkage map containing PCR and RFLP markers
• Factors influencing efficiency of genetic mapping projects

 

The following might be useful - Molecular Biology WebBook and The Glossary of Biotechnology Terms.

A company providing many of these techniques in the service of agriculture is Biogenetic Services, Inc.

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